Isolation and characterization of bacteria tolerant to organic solvent for their exploitation in bioremediation
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Date
2012-02-13T06:30:54Z
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Abstract
Total twenty five organic solvent tolerant bacteria (OSTB) have been isolated
from six different sites. Polyphasic approach was employed for identification of
OSTB including morphological and biochemical characterization, fatty acid profiling
and 16S rRNA gene sequencing. Amongst them, twenty belonged to Bacillus spp.,
two to Micrococcus sp., and one each of Geobacillus sp., Terribacillus sp., and
Staphylococcus sp. Thus, Bacillus group of species are dominating in this study. With
this approach we could identify 19 isolates up to species level while six OSTB could
be identified only up to genus level (Bacillus). Hence, future study in this direction
using the latest techniques like DNA-DNA hybridization would lead to proper
identification of these isolates and might help in establishing the relationship with
known Bacillus spp.
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Microbiology